transfection 141 intestinal epithelium cell 6  (ATCC)

Journal: Bioactive Materials

Article Title: Integrated fabrication of a shape-adaptable, antioxidative composite stent for effective closure and biological repair of enteroatmospheric fistula

doi: 10.1016/j.bioactmat.2026.01.014

Figure Lengend Snippet: Biocompatibility and cell migration of the composite stent. (A) Live/dead staining of IEC-6 cells cultured with the stent, showing cell viability. (B) Hemocompatibility of the stent: (i) hemolysis assay of red blood cells, (ii) corresponding hemolysis percentage. (C) Representative images of cell migration in the scratch assay. (D) Quantification of (i) migration area and (ii) percentage closure over time. (E) Representative Transwell images of migrated cells. (F) Quantification of migrated cells in the Transwell assay. Data were presented as mean ± SD (n = 3). ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001. Gel represents the G 2 S 4 hydrogel group, and Comp represents the composite stent group; the same abbreviations are used in the following figures.

Article Snippet: IEC-6 cells and RAW264.7 cells were purchased from KeyGEN BioTech (Nanjing, China).

Techniques: Migration, Staining, Cell Culture, Hemolysis Assay, Wound Healing Assay, Transwell Assay

Journal: Bioactive Materials

Article Title: Integrated fabrication of a shape-adaptable, antioxidative composite stent for effective closure and biological repair of enteroatmospheric fistula

doi: 10.1016/j.bioactmat.2026.01.014

Figure Lengend Snippet: Antioxidant and anti-inflammatory effects of the composite stent. (A) ROS levels in RAW264.7 and IEC-6 cells by DCFH-DA staining. (B, C) Quantification of ROS fluorescence by integrated density. (D) Mitochondrial ROS detected by MitoSOX staining. (E, F) Quantification of mitochondrial ROS by integrated density. (G) Co-culture system of the composite stent with macrophages using Transwell chambers. (H) Flow cytometry analysis of macrophage polarization based on CD86 (M1 marker) and CD206 (M2 marker) expression under different stimuli. (I) Quantitative analysis of CD86 + macrophages obtained from flow cytometry. (J–L) ELISA measurements of pro-inflammatory cytokines (J) TNF-α, (K) IL-6, and (L) IFN-β in the culture supernatant. Data were presented as mean ± SD (n = 3). ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001.

Article Snippet: IEC-6 cells and RAW264.7 cells were purchased from KeyGEN BioTech (Nanjing, China).

Techniques: Staining, Fluorescence, Co-Culture Assay, Flow Cytometry, Marker, Expressing, Enzyme-linked Immunosorbent Assay

Journal: bioRxiv

Article Title: CDK4/6 inhibitors enhance oxaliplatin efficacy in colorectal cancer with RB-dependent and tumor-selective activity in intestinal model

doi: 10.64898/2026.04.15.718743

Figure Lengend Snippet: (A) Representative fluorescence images of IEC-6 cells treated for 48 h with oxaliplatin (0.6 μM), abemaciclib (300 nM), palbociclib (400 nM), or their combinations. Viable cells were detected by Calcein AM fluorescence (green), and nuclei were counterstained with Hoechst (blue). Scale bar: 1000 μm. (B) Quantification of Calcein AM–positive cells following treatment with oxaliplatin and abemaciclib. (C) Quantification of Calcein AM–positive cells following treatment with oxaliplatin and palbociclib. (D) Quantification of ethidium homodimer–positive cells in IEC-6 cultures treated with oxaliplatin and abemaciclib. (E) Quantification of ethidium homodimer–positive cells in IEC-6 cultures treated with oxaliplatin and palbociclib. (F) A summary table showing the percentage of viable cells in HCT116 (tumor) and IEC-6 (non-tumor) cell lines across treatments. The data are presented as the mean ± SEM of three independent biological replicates. Quantification was performed by counting Calcein AM–positive cells relative to total nuclei. Statistical analysis was performed using one-way ANOVA followed by Šídák’s multiple comparisons test. Significance levels are indicated as follows: *p < 0.05; **p < 0.01; ***p < 0.001.

Article Snippet: The non-tumoral rat intestinal epithelial cell line IEC-6 (ATCC: CRL-1592), kindly donated by Prof. José Garcia Abreu (ICB/UFRJ), was maintained in the same basal medium (DMEM/F-12 supplemented with 10% FBS) with additional insulin supplementation at a final concentration of 0.1 U/mL throughout all experimental procedures, including treatments.

Techniques: Fluorescence

Journal: Life

Article Title: Spermine Ameliorates DSS-Induced Ulcerative Colitis in Mice by Improving Mitophagy and Intestinal Microbiota

doi: 10.3390/life16030417

Figure Lengend Snippet: Spe cytotoxicity evaluation on IEC-6 cells. The effects of different concentrations of Spe (0, 2.5, 5, 7.5 μM) on IEC-6 cell viability were assessed using the CCK-8 assay. Data are presented as mean ± SEM. * p < 0.05 vs. control group.

Article Snippet: IEC-6 cells were obtained from Servicebio (Wuhan, China) and maintained in DMEM high-glucose medium supplemented with 5–10% fetal bovine serum and 1% penicillin/streptomycin at 37 °C under 5% CO 2 .

Techniques: CCK-8 Assay, Control

Journal: Life

Article Title: Spermine Ameliorates DSS-Induced Ulcerative Colitis in Mice by Improving Mitophagy and Intestinal Microbiota

doi: 10.3390/life16030417

Figure Lengend Snippet: Effects of mitophagy inhibition on LPS-induced mitochondrial damage in IEC-6 cells. ( A , B ) Mitochondrial membrane potential was detected using the JC-1 staining method. ( C , D ) Assessment of mitochondrial reactive oxygen species levels. Data are presented as mean ± SEM. ** p < 0.01, *** p < 0.001 vs. control group. ## p < 0.01 vs. DSS group.

Article Snippet: IEC-6 cells were obtained from Servicebio (Wuhan, China) and maintained in DMEM high-glucose medium supplemented with 5–10% fetal bovine serum and 1% penicillin/streptomycin at 37 °C under 5% CO 2 .

Techniques: Inhibition, Membrane, Staining, Control

Journal: Frontiers in Pharmacology

Article Title: Hydroxysafflor yellow A attenuates sepsis-induced intestinal barrier dysfunction by modulating Bcl-2/SOD2-mediated mitochondrial apoptosis

doi: 10.3389/fphar.2026.1728183

Figure Lengend Snippet: Proteomic Analysis for Identifying Key Molecules Mediating Ferroptosis in Sepsis-Associated IEC-6 Cells (A) KEGG pathway enrichment analysis. (B) Immunofluorescence staining of ZO-1 (scale bar = 50 μm) and Western blot analysis of ZO-1 expression. (C) CCK-8 assay of cell viability (n = 8). (D) DCFH-DA showing ROS levels (scale bar = 20 μm, n = 8) (E) TUNEL staining of apoptotic cells. (F) Ki-67 immunofluorescence of proliferating cells (G) MitoTracker Red CMXRos staining showing mitochondrial morphology (scale bar = 50 μm, n = 3). (H) TEM images of mitochondrial ultrastructure. (I) Impact of LPS on mitochondrial respiration in IEC-6 cells (n = 3).

Article Snippet: The rat intestinal epithelial cell line IEC-6 (ATCC, USA) was maintained in Dulbecco’s Modified Eagle Medium (DMEM; Hyclone, USA) supplemented with 10% fetal bovine serum (FBS; Hyclone, USA) under standard conditions (37 °C, 5% CO 2 , humidified incubator).

Techniques: Immunofluorescence, Staining, Western Blot, Expressing, CCK-8 Assay, TUNEL Assay

Journal: Frontiers in Pharmacology

Article Title: Hydroxysafflor yellow A attenuates sepsis-induced intestinal barrier dysfunction by modulating Bcl-2/SOD2-mediated mitochondrial apoptosis

doi: 10.3389/fphar.2026.1728183

Figure Lengend Snippet: Molecular Docking, Molecular Dynamics Analysis, and in vitro / in vivo Validation of Key Proteins. (A) Chemical structure of HSYA. (B) Venn diagram integrating sepsis-, HSYA-, and apoptosis-related targets, highlighting Bcl-2 and SOD2 as key molecules. (C) Molecular docking and molecular dynamics analysis showing binding interactions of HSYA with Bcl-2. (D) Molecular docking and molecular dynamics analysis showing binding interactions of HSYA with SOD2. (E) Western blot analysis of Bcl-2 and SOD2 protein expression in IEC-6 cells (n = 3). (F) Western blot analysis of Bcl-2 and SOD2 protein expression in intestinal tissues (n = 3).

Article Snippet: The rat intestinal epithelial cell line IEC-6 (ATCC, USA) was maintained in Dulbecco’s Modified Eagle Medium (DMEM; Hyclone, USA) supplemented with 10% fetal bovine serum (FBS; Hyclone, USA) under standard conditions (37 °C, 5% CO 2 , humidified incubator).

Techniques: In Vitro, In Vivo, Biomarker Discovery, Binding Assay, Western Blot, Expressing

Journal: Frontiers in Pharmacology

Article Title: Hydroxysafflor yellow A attenuates sepsis-induced intestinal barrier dysfunction by modulating Bcl-2/SOD2-mediated mitochondrial apoptosis

doi: 10.3389/fphar.2026.1728183

Figure Lengend Snippet: In vitro effects of HSYA on IEC-6 cells following Bcl-2 inhibition. (A) Western blot analysis showing the relative expression level of Bcl-2 after treatment in Si-Bcl-2 of IEC-6 cells (n = 3). (B) Western blot analysis showing the effects of HSYA + Si-Bcl2 on the expression of Bcl2 in LPS-stimulated IEC-6 cells (n = 3). (C) TUNEL staining of apoptotic cells (scale bar = 50 μm, n = 3). (D) Ki-67 immunofluorescence for proliferating cells. (E,F) DCFH-DA (n = 3) and MitoTracker Red CMXRos staining (n = 8) showing intracellular ROS levels and mitochondrial morphology in IEC-6 cells (scale bar = 20 μm). (G) TEM images showing mitochondrial ultrastructure in IEC-6 cells following HSYA and HSYA + Si-Bcl-2 treatment. (H) Impact of HSYA + Si-Bcl-2 treatment on mitochondrial respiration in LPS-stimulated IEC-6 cells (n = 3).

Article Snippet: The rat intestinal epithelial cell line IEC-6 (ATCC, USA) was maintained in Dulbecco’s Modified Eagle Medium (DMEM; Hyclone, USA) supplemented with 10% fetal bovine serum (FBS; Hyclone, USA) under standard conditions (37 °C, 5% CO 2 , humidified incubator).

Techniques: In Vitro, Inhibition, Western Blot, Expressing, TUNEL Assay, Staining, Immunofluorescence

Journal: Frontiers in Pharmacology

Article Title: Hydroxysafflor yellow A attenuates sepsis-induced intestinal barrier dysfunction by modulating Bcl-2/SOD2-mediated mitochondrial apoptosis

doi: 10.3389/fphar.2026.1728183

Figure Lengend Snippet: Effects of HSYA on intestinal epithelial cells under silence SOD-2. (A,B) Western blot analysis of SOD2 protein expression in IEC-6 cells (n = 3). (C) TUNEL staining for apoptotic cells (scale bar = 50 μm). (D) Ki-67 immunofluorescence for proliferating cells (scale bar = 50 μm, n = 8). (E,F) DCFH-DA (n = 8)and MitoTracker Red CMXRos staining (n = 3) showing intracellular ROS accumulation and mitochondrial morphology in IEC-6 cells (scale bar = 20 μm). (G) TEM images showing mitochondrial ultrastructure in IEC-6 cells treated with HSYA or HSYA + Si-SOD2 (scale bar = 1 μm). (H) Impact of HSYA + Si-SOD2 treatment on mitochondrial respiration in LPS-stimulated IEC-6 cells (n = 3).

Article Snippet: The rat intestinal epithelial cell line IEC-6 (ATCC, USA) was maintained in Dulbecco’s Modified Eagle Medium (DMEM; Hyclone, USA) supplemented with 10% fetal bovine serum (FBS; Hyclone, USA) under standard conditions (37 °C, 5% CO 2 , humidified incubator).

Techniques: Western Blot, Expressing, TUNEL Assay, Staining, Immunofluorescence